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The relevance of α‐naphthyl acetate esterases to various monocyte functions
Author(s) -
Oertel J.,
Hagner G.,
Kastner M.,
Huhn D.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb02886.x
Subject(s) - cytotoxicity , antibody dependent cell mediated cytotoxicity , monocyte , lytic cycle , k562 cells , phagocytosis , cell culture , chemistry , microbiology and biotechnology , agarose , in vitro , biology , biochemistry , immunology , virus , genetics
S ummary . Human monocytes contain a series of α‐naphthyl acetate (αNA) esterases which are not present in other blood cells and which can be specifically inhibited by bis(4‐nitrophenyl)‐phosphate (BNPP). This inhibitor is non‐toxic at the concentration used and thus enabled studies on the possible significance of this enzyme towards various monocyte functions. BNPP has no noticeable influence on adhesion and spreading of monocytes on glass surfaces, nor does it inhibit the phagocytosis of IgG‐coated latex particles. BNPP does, however, diminish the spontaneous cytotoxicity of freshly isolated monocytes towards the erythroleukaemic cell line K562. In the single cell assay in agarose, BNPP treatment of monocytes leads to a decrease in the number of lytic and non‐lytic effector‐target cell conjugates. In contrast, the antibody‐dependent cell‐mediated cytotoxicity (ADCC) of monocytes as well as the natural cytotoxicity of lymphocytes towards K562 cells are not influenced by BNPP. The present investigations show that monocyte specific αNA esterases are involved in the spontaneous cytotoxicity of monocytes toward tumour cells.