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Inhibitory effect of PWM‐stimulated OKT4 + subsets on erythro‐, granulo‐ and megakaryocytopoiesis in vitro
Author(s) -
Sakamaki Hisashi,
Hamaguchi Hiroyuki,
Furusawa Shimpei,
Shishido Hideo
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb02877.x
Subject(s) - pokeweed mitogen , megakaryocytopoiesis , haematopoiesis , microbiology and biotechnology , t cell , erythropoiesis , bone marrow , monoclonal antibody , stimulation , in vitro , biology , megakaryocyte , immunology , immune system , endocrinology , stem cell , antibody , medicine , concanavalin a , biochemistry , anemia
S ummary . Normal human peripheral blood T cells and T‐cell subsets defined by monoclonal antibodies of the OKT series were pretreated with pokeweed mitogen (PWM). Their effects on the haematopoietic precursors, erythroid (BFU‐E, CFU‐E), granulocyte‐macrophage (CFU‐GM) and megakaryocyte (CFU‐M) colony forming cells were evaluated by coculture. While unstimulated T cells and T‐cell subsets enhanced growth of autologous blood BFU‐E, PWM‐stimulated T and OKT4 + cells suppressed it, also inhibiting proliferation of both autologous and allogeneic bone marrow BFU‐E, CFU‐E, CFU‐GM and CFU‐M. PWM‐stimulated OKT8 + cells had little effect on the growth of any of the precursors at the cell concentration at which growth was completely inhibited by PWM‐stimulated OKT4 + cells. Irradiation of T or OKT4 + cells with 3000 rad before PWM stimulation completely abrogated the inhibition. These observations might be related to the mechanism of pancytopenia in some cases of immune‐mediated aplastic anaemia.