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Membrane characteristics and osmotic fragility of red cells, fractionated with anglehead centrifugation and counterflow centrifugation
Author(s) -
Van Der Vegt S. G. L.,
Ruben A. M. Th.,
Werre J. M.,
Gier J.,
Staal G. E. J.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb02844.x
Subject(s) - centrifugation , tonicity , erythrocyte fragility , phospholipid , chromatography , chemistry , membrane , volume (thermodynamics) , hemolysis , biochemistry , biology , immunology , physics , quantum mechanics
S ummary . Red cell populations were separated on the basis of differences in density using anglehead centrifugation and on the basis of differences in mean cell volume using counterflow centrifugation. In the different fractions, mean surface area was calculated, phospholipid and cholesterol content determined as well as the osmotic behaviour in hypotonic salt solutions. Older red cells appeared to be more resistant to hypotonic salt solutions, due to favourable surface area to volume ratio.