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Lineage specific classification of leukaemia: results of the analysis of sixty cases of childhood leukaemia
Author(s) -
Ha Kyungsae,
Hozumi Nobumichi,
Hrincu Angela,
Gelfand Erwin W.
Publication year - 1985
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1985.tb02822.x
Subject(s) - calla , gene rearrangement , acute lymphocytic leukemia , southern blot , lineage (genetic) , biology , gene , leukemia , antibody , immunology , genetics , monoclonal antibody , lymphoblastic leukemia
S ummary . In addition to conventional morphological, histochemical and immunological marker studies, cells from 60 children with leukaemia were further analysed using the Southern blot hybridization technique to look at differences in the organization of immunoglobulin (Ig) genes. Of the 60 patients studied by conventional means, 47 were diagnosed as acute lymphocytic leukaemia (ALL) and 13 as non‐lymphocytic leukaemia. Seven patients were initially classified as T ALL and 40 as non‐T, non‐B ALL. Further subclassification of the 40 patients with non‐T, non‐B ALL indicated three pre‐B ALL and 29 patients diagnosed as common ALL, expressing Ia and CALLA antigens. All 29 patients with common ALL demonstrated Cμ gene rearrangements with or without light chain (κ and λ) genes rearrangement. Based on the developmental hierarchy of Ig gene rearrangements, it was possible to further subclassify the patients with common ALL into different stages of B cell development. Eight (of the 40) patients with non‐T, non‐B ALL were identified as CALLA ‐ but further analysis indicated T‐lineage origin in two patients and three patients were reclassified as acute undifferentiated leukaemia (AUL). Cμ gene rearrangements were detected in two patients with T ALL, two patients with AUL and one patient with acute myelogenous leukaemia (AML). In contrast to the patients with common ALL, Ig gene rearrangement observed in these non‐B‐lineage cells was restricted to a single Cμ gene while retaining germ‐line configuration of the other allele of the Cμ gene and both light chain genes.