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The mechanism of the anaemia in rheumatoid arthritis: effects of bone marrow adherent cells and of serum on in‐vitro erythropoiesis
Author(s) -
Reid C. D. L.,
Prouse P. J.,
Baptista L. C.,
GUMPEL J. M.,
Chanarin I.
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb06107.x
Subject(s) - bone marrow , erythropoiesis , medicine , rheumatoid arthritis , ineffective erythropoiesis , immunology , anemia , endocrinology , in vitro , progenitor cell , biology , stem cell , genetics , biochemistry
Summary Clonal assays for erythroid progenitors (BFU‐e and CFU‐e) were used to study 20 patients with rheumatoid arthritis, 15 of whom had anaemia of chronic disease and five of whom were haematologically normal. The numbers of bone marrow BFU‐e and CFU‐e in the anaemic patients did not differ significantly from those in normal controls. Macrophages were removed from the bone marrow by a combination of adherence and buoyant density centrifugation over a sucrose gradient and the resulting fractions were cultured alone or together with autologous adherent cells in BFU‐e assays. Co‐culture with adherent cells significantly increased colony growth in both the controls and in seven of eight anaemic patients studied. Serum from 14 anaemic patients and from five non‐anaemic patients was added to cultures of bone marrow or to control peripheral blood ‘null’ cells. Anaemic serum uniformly either inhibited or failed to stimulate BFU‐e growth under these conditions. Serum from non‐anaemic patients and from 10 healthy controls stimulated BFU‐e growth from ‘null’ cells to an equal degree.