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Monoclonal antibody specific for granulocytic‐lineage cells and reactive with human pluripotent and committed haematopoietic progenitor cells
Author(s) -
JanowskaWieczorek Anna,
Mani Patrice,
Turner A. Robert,
McGann Locksley E.,
Shaw Andrew R. E.,
Turc JeanMichel
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb06070.x
Subject(s) - haematopoiesis , monoclonal antibody , progenitor cell , microbiology and biotechnology , bone marrow , biology , antibody , antigen , granulocyte , immunology , monocyte , lymphoblast , megakaryocyte , megakaryocytopoiesis , cell culture , stem cell , genetics
Summary A murine monoclonal antibody (82H5, IgM class) has been developed that detects an antigenic determinant expressed by ± 90% of normal granulocytes and 60‐80% of light‐density normal bone‐marrow cells, including human pluri‐potential progenitors (colony‐forming‐unit‐granulocyte, erythroid, macrophage, megakaryocyte; CFU‐GEMM) and committed progenitors: granulocyte‐macrophage (CFU‐GM), erythroid (BFU‐E), and megakaryocytic (CFU‐MK). This antibody did not react with erythrocytes, monocytes, platelets, lymphocytes from normal peripheral blood, lymphoblasts from patients with acute lymphoblastic leukaemia, or with lymphoid cell lines. The 82H5‐defined antigenic determinant was expressed on ±90% of leukaemic cells of promyelocytic, myelomonocytic and monocytic morphology, and cell lines KG.1, ML.1, HL.60, K562 and U.937. Cortical thymocytes were unreactive with 82H5. Treatment of human bone‐marrow cells with granulocytic‐specific monoclonal antibody 82H5 plus complement significantly inhibited colony formation (48‐74%; P±0.05) of CFU‐GEMM, CFU‐GM, BFU‐E, CFU‐MK, whereas treatment with control monoclonal anti‐la antibody plus complement caused 79‐89% inhibition. This antibody reacted strongly with 3‐fuc‐NAc lactosamine when tested with a panel of synthetic carbohydrate structures. We conclude that 82H5 may be a useful probe for phenotypic analysis of leukaemic cells and investigation of haematopoiesis.