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Conversion of endogenous cobalamins into microbiologically‐inactive cobalamin analogues in rats by exposure to nitrous oxide
Author(s) -
Muir M.,
Chanarin I.
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb03999.x
Subject(s) - cobalamin , chemistry , nitrous oxide , endogeny , in vivo , cyanocobalamin , biochemistry , vitamin b12 , biology , organic chemistry , microbiology and biotechnology
S ummary . Rats were exposed to a mixture of oxygen and nitrous oxide (1/1) for up to 18 d. There was a marked fall in both the serum and liver cobalamin levels. There was a significant rise in the concentration of microbiologically‐inactive cobalamins in serum 24 h after exposure to N 2 0. The proportion of microbiologically‐inactive cobalamin in liver increased throughout the period of exposure. We were unable to show a similar effect in sera from patients inhaling N 2 0 for 24 h, nor by exposing rat plasma to N 2 O for 24 h. It was concluded that microbiologically‐inactive cobalamins are produced in vivo from endogenous cobalamins and that one form appears to be a product of cobalamin oxidation.