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Chromosome analysis of isolated colony erythroblasts in chronic myelogenous leukaemia
Author(s) -
Dainiak Nicholas,
Liu Aileen,
Dewey Maureen C.,
Kulkarni Vivekanand
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb03980.x
Subject(s) - karyotype , basophilic , bone marrow , biology , chromosome analysis , microbiology and biotechnology , progenitor cell , erythropoietin , cytogenetics , immunology , chromosome , erythropoiesis , pathology , stem cell , cancer research , andrology , genetics , medicine , anemia , gene
S ummary . To isolate and karyotype the progeny of erythroid progenitors, we applied colony erythroblasts derived from plasma clot marrow cultures from two healthy adults and two patients with newly diagnosed Ph 1 + chronic myelogenous leukaemia (CML) to discontinuous Stractan density gradients. Erythroid colony proliferation by patient cells was increased relative to that of normal donor cells ( P <0.01). ‘Endogenous’colonies appeared in patient but not in normal donor marrow cultures. Greater than 95% of nucleated cells equilibrating at 1.071 were basophilic proerythroblasts. While analysis of chromosome spreads of normal donor cells in this fraction showed normal karyotypes, cells from patient marrow cultures were Ph 1 +, whether cultured in the presence or absence of added erythropoietin. These findings suggest that chromosome abnormalities of erythroid progenitors may be expressed by their progeny in tissue culture, and that Stractan may be a useful supporting medium for separating colony erythroblasts for chromosome analysis.