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Evidence for clonal evolution in pre‐B‐cell leukaemia
Author(s) -
Abromowitch Minnie,
Williams Dorothy L.,
Melvin Susan L.,
Stass Sanford
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb03971.x
Subject(s) - calla , karyotype , chromosomal translocation , biology , immunophenotyping , somatic evolution in cancer , cytogenetics , phenotype , lymphoblast , ploidy , genetics , microbiology and biotechnology , chromosome , cell culture , flow cytometry , cancer , gene , antibody , monoclonal antibody
S ummary . This case study provides evidence for clonal evolution in pre‐B‐cell leukaemia. At diagnosis, the lymphoblasts from a 3‐year‐old boy were morphologically subtyped as L1 (French–American–British classification). Their immunophenotype was CALLA + , CIgM + , SIg ‐ , TdT + , and the karyotype was pseudodiploid with a 1;19 translocation. Striking shifts were apparent when the child relapsed 16 months later. The morphologic subtype had changed to L3, CALLA and TdT had disappeared, and a consistent karyotype was lacking. The modal chromosome number had increased through clonal evolution to 85, the 1;19 translocation was retained, and a new marker, a 14q + (partial duplication) appeared and was present in a majority of cells. These cytogenetic findings are characteristic of a transforming state. However, despite the loss of TdT, the appearance of classic L3 morphology and the acquisition of a 14q + marker, the cells retained a predominantly pre‐B phenotype.