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Effect of pure erythropoietin on DNA‐synthesis by human marrow day 15 erythroid burst forming units in short‐term liquid culture
Author(s) -
Dessypris Emmanuel N.,
Krantz Sanford B.
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb03957.x
Subject(s) - erythropoietin , incubation , dna synthesis , erythropoiesis , microbiology and biotechnology , bone marrow , cell culture , thymidine , chemistry , medicine , andrology , endocrinology , biology , dna , immunology , biochemistry , anemia , genetics
S ummary . The effect of pure erythropoietin (EP) on human marrow day 15 burst‐forming units‐erythroid (BFU‐E) was studied using a short‐term liquid culture system containing 30% human serum. Non‐adherent marrow cells were cultured in liquid medium for 0–48 h and then the number of BFU‐E was assayed by the use of the plasma clot method. The addition of 1 U/ml of EP into the liquid culture medium resulted in maintenance of the number of BFU‐E assayed after 24–48 h of incubation. The number of BFU‐E recovered after 24–48 h culture was directly proportional to the concentration of EP present in the liquid medium. In addition, the proliferative status of BFU‐E before and after exposure to EP was studied by 3 H‐thymidine and hydroxyurea suicide. It was found that EP doubles the percentage of BFU‐E in DNA synthesis after 24–48 h of incubation in the liquid medium. This effect of EP on DNA synthesis by bone marrow day 15 BFU‐E is detectable as early as 6 h after the onset of incubation and at EP concentrations as low as 0.2 U/ml of medium, a concentration present in the serum of moderately anaemic patients. The human marrow day 15 BFU‐E is an EP‐responsive cell and pure EP can induce it into DNA synthesis.