Premium
Separation of diploid from aneuploid cells in acute lymphoblastic leukaemia
Author(s) -
Walle A. J.,
Niedermayer W.
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb02934.x
Subject(s) - flow cytometry , ploidy , bone marrow , biology , immunology , lymphoblastic leukemia , aneuploidy , separation (statistics) , cell , microbiology and biotechnology , pathology , leukemia , medicine , genetics , gene , machine learning , computer science , chromosome
S ummary. A new method for the separation of diploid and aneuploid cells from blood of patients with leukaemia is described. Density gradient separation using a modified silica particle gradient was combined with analysis of cellular DNA content by flow cytometry. Submicroscopic levels of diploid or aneuploid cells were enriched up to 90% purity. Three patients with acute lymphoblastic leukaemia and no evidence of aneuploid cells after separation are in continuing complete remission after 24.5–47 months. Two patients with aneuploid cells detected after separation expired in relapse 0.5 and 3 months later. Cell separation prior to flow cytometric analysis might be a useful tool to detect submicroscopic levels of aneuploid leukaemic cells in blood and bone marrow, and to predict impending relapse.