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Kinetic studies of the activation of factor X by factors IXa and VIII:C in the absence of thrombin
Author(s) -
Neal Gregory G.,
Esnouf M. Peter
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb02872.x
Subject(s) - factor x , factor ixa , thrombin , coagulation , frequency factor , factor v , chemistry , factor ix , tissue factor , thrombin generation , kinetic energy , microbiology and biotechnology , biochemistry , activation energy , medicine , biology , platelet , physics , quantum mechanics , thrombosis
S ummary. The activation of factor X by the components of the intrinsic coagulation pathway has been studied using a preparation of bovine factor VIII which did not require ‘activation’ with thrombin prior to its participation in the reaction. Measurements of the kinetic parameters indicated that factor VIII: C stimulated the formation of factor X a via a 170‐fold increase in the V max ; factor VIII: C had little effect on the K m for factor X.