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Immunophenotyping of leukaemia: an immunoperoxidase method using air‐dried smears
Author(s) -
Sandhaus Linda M.,
GajlPeczalska Kazimiera J.,
Brunning Richard D.
Publication year - 1984
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1984.tb01278.x
Subject(s) - immunoperoxidase , immunophenotyping , medicine , pathology , immunology , flow cytometry , antibody , monoclonal antibody
S ummary An immunoperoxidase (IP) method utilizing monoclonal antibodies to demonstrate the presence of the common acute lymphocytic leukaemia antigen (CALLA) on leukaemic cells using air‐dried blood and bone marrow smears is described. Two readily available CALLA‐specific monoclonal antibodies, BA‐3 and J5, and an avidin‐biotin immunoperoxidase methodology were utilized. Nineteen cases of acute leukaemia or blast crisis of chronic myelogenous leukaemia were studied. Immunofluorescent studies correlated closely with the results of the immunoperoxidase technique.