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Allogeneic bone marrow transplantation: the monitoring of granulocyte macrophage colonies following the collection of bone marrow mononuclear cells and after the subsequent in‐vitro cytolysis of OKT3 positive lymphocytes
Author(s) -
Gilmore M. J. M. L.,
Prentice H. G.,
Jones E. Price,
Blacklock H. A.,
Tidman N.,
Schey S.,
Goldstein G.,
Janossy G.,
Hoffbrand A. V.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb02840.x
Subject(s) - peripheral blood mononuclear cell , bone marrow , ficoll , immunology , granulocyte , transplantation , monoclonal antibody , medicine , cytolysis , lymphocyte , in vitro , microbiology and biotechnology , biology , antibody , cytotoxic t cell , biochemistry
S ummary. Marrow nucleated cells from eight normal allogeneic donors was layered on Ficoll‐Metrizoate to isolate the mononuclear cell fraction. The cells were then washed to remove Ficoll‐Metrizoate and coagulation factors prior to resuspension in a balanced salt solution and the addition of the murine anti‐human T‐lymphocyte monoclonal antibody OKT3 and rabbit complement. The procedures were assessed for their effect on mononuclear cell viability (mean recovery 84·4%); the ability of the cells to proliferate granulocyte‐macrophage colonies (mean recovery 57·4%); the in‐vitro T‐lymphocytolysis (mean 75·7%) and the removal of rabbit complement (> 99%). Following marrow transplantation with this treated mononuclear fraction the mean day to recovery of ≥ 1·0 × 10 9 /1 leucocytes was 20 d, with three patients developing ≥ Grade II acute graft versus host disease (GvHD). Thus, treatment of donor marrow with OKT3 and complement in a large volume system was not detrimental to subsequent engraftment, nor effective in complete T‐lymphocytolysis, nor in prevention of severe GvHD.