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Preservation of deformability (filterability) of sickle cells by BW12C during progressive deoxygenation
Author(s) -
Kenny M. W.,
Stuart J.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb02161.x
Subject(s) - deoxygenation , in vitro , venous blood , chemistry , red blood cell , biophysics , medicine , biochemistry , biology , catalysis
S ummary . venous blood from patients with sickle‐cell disease in the steady state or in crisis was progressively deoxygenated in vitro to study the effect of BW12C, a new compound designed to stabilize haemoglobin in the oxy‐conformation, on the deformability (filterability) of washed erythrocytes. At a final concentration of 1·5 mM, BW12C significantly increased erythrocyte deformability, compared with no added compound, at all levels of deoxygenation below normal arterial PO 2 . At concentrations of 3·0 and 5·0 mM, BW12C prevented any significant reduction of erythrocyte deformability, or increase in sickled cells, with deoxygenation down to PO 2 values below the normal venous level. These in vitro results demonstrate the considerable potential, as an anti‐sickling agent, of this novel compound.