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Interferon production by human marrow stromal cells
Author(s) -
Shah Girish,
Dexter T. Michael,
Lanotte Michel
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb02111.x
Subject(s) - stromal cell , cell culture , bone marrow , biology , interferon , cell , interferon gamma , cancer research , immunology , cytokine , biochemistry , genetics
S ummary . We have demonstrated that normal human bone marrow stromal cells can be induced to produce high levels of β‐interferon (IFN). One of three randomly selected human stromal cell lines produced β‐IFN in similar amounts to one of the best β‐IFN producer cell lines (MG‐63 osteosarcoma cells). The marrow stromal cell lines did not produce γ ‐IFN, though a low level of α‐IFN was apparently produced. The stromal cell lines differ from usual producer cell lines in that they can be subcultured for a much longer growth period and also maintain the ability to producer IFN. The cells are karyotypically normal and are not virus transformed. Such cell lines may be useful in the production of human IFN as well as allowing studies on the role of IFN in stromal cell‐haemopoietic cell interactions.