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Influence of the tumour promoter TPA upon erythroid burst formation in vitro
Author(s) -
Brockbank K. G. M.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb02049.x
Subject(s) - in vitro , bone marrow , population , chemistry , microbiology and biotechnology , incubation , inhibitory postsynaptic potential , endocrinology , medicine , biology , immunology , biochemistry , environmental health
S ummary . A dose dependent effect of the tumour promoter TPA on burst formation by rabbit erythroid progenitors (BFU‐e) was demonstrated in cultures deficient in the early erythroid regulator burst‐promoting activity (BPA). In these culture conditions the burst number was highest (193% of controls) at 10 9 m TPA and concentrations higher than 3 × 10 9 m TPA were inhibitory. The degree of burst enhancement by TPA and bone marrow conditioned medium as a source of BPA was similar. The addition of optimal concentrations of both TPA and BPA simultaneously to cultures resulted in no further increase in burst number. Short‐term incubation of bone marrow cells with TPA failed to enhance the percentage of S‐phase BFU‐e under conditions in which BPA significantly increases the number of BFU‐e in the cell cycle. These results indicate that the same population of BFU‐e responds to TPA and BPA, but TPA does not mimic the mitogenic effect of BPA upon BFU‐e.