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Solid‐phase vitamin B 12 assays using polyacrylamide‐bound intrinsic factor and polyacrylamide‐bound R‐binder
Author(s) -
Muir M.,
Chanarin I.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb02043.x
Subject(s) - polyacrylamide , vitamin b12 , intrinsic factor , polyacrylamide gel electrophoresis , phase (matter) , cyanocobalamin , chemistry , chromatography , biochemistry , polymer chemistry , organic chemistry , enzyme
S ummary . A new solid phase vitamin B 12 assay is described using intrinsic factor to measure microbiologically‐available B 12 and R‐binder to measure total B 12 . The solid phase reagent consists of intrinsic factor coupled to polyacrylamide beads and salivary R‐binder coupled to polyacrylamide beads. The assay is simple to perform and separates completely sera from controls and patients with megaloblastic anaemia due to B 12 deficiency.