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Enrichment of erythroblasts from human bone marrow using complement‐mediated lysis: measurement of ferritin
Author(s) -
Ali Faieza M. K.,
May Alison,
Jones Brian M.,
Jacobs Allan
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb02015.x
Subject(s) - ferritin , bone marrow , lysis , complement (music) , iron isotopes , human bone , erythroblast , immunology , medicine , pathology , cancer research , erythropoiesis , biology , anemia , biochemistry , phenotype , in vitro , isotope , physics , quantum mechanics , complementation , gene
S ummary . Sequential lysis of human bone marrow cells with a monoclonal antibody directed against myeloid cells (TG1) and a rabbit antiserum raised against peripheral blood mononuclear cells gave preparations in which 78–97% of the nucleated cells were erythroid, with a 24–77% recovery. Viability was high, morphology was good and the cells were able to divide and differentiate in culture. No metabolic experiments were carried out but the ferritin content of the erythroblasts was measured in four experiments and found to be about 200–2000 times higher than that found in normal erythrocytes. The H/S ratio was high in both erythroblasts and erythrocytes. Fractionation on the basis of density of two erythroblast preparations, one from a patient with sideroblastic anaemia and one from a patient with megaloblastic anaemia, showed that the most immature erythroblasts contained the highest content of ferritin and that this fell with maturation. The H/S ratio stayed the same or fell with maturation. It was concluded that this method would be valuable for the study of the role of erythroblast ferritin in normal and pathological situations.