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Studies on the stability of factor VIII coagulant antigen (VIII: CAg) in the presence of VIII: C antibodies
Author(s) -
Furlong R. A.,
Peake I. R.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb01986.x
Subject(s) - serial dilution , antibody , chemistry , immunoradiometric assay , antigen , in vitro , incubation , microbiology and biotechnology , isoantibodies , chromatography , radioimmunoassay , biochemistry , immunology , medicine , biology , alternative medicine , pathology
S ummary . The stability of factor VIII coagulant antigens (VIII:CAg) at 56°C was investigated using an immunoradiometric assay for VIII: CAg. In normal or CRM + haemophilic plasmas VIII: CAg was rapidly inactivated at 56°C. VIII: CAg in spontaneous VIII: C inhibitor plasmas and in post‐treatment samples from haemophiliacs with VIII: C inhibitor was resistant to inactivation at 56°C, indicating the presence of heat stable VIII: CAg‐anti VIII: CAg complexes. In vitro VIII: CAg‐anti VIII: CAg complexes were formed by incubation of diluted VIII: C antibodies and normal plasma and the stability of these complexes at 56°C was studied. Haemophilic VIII: CAg antibodies formed heat stable immune complexes over a narrow range of inhibitor dilutions whilst some spontaneous VIII: CAg antibodies formed these stable complexes over a much wider range of dilutions emphasizing the difference in the properties of these antibodies.