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Granulocyte‐macrophage progenitor cell preservation at 4°C
Author(s) -
Delforge Alain,
RongeCollard Elisabeth,
Stryckmans Pierre,
Spiro Théodore,
Malarme Marie A.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb01985.x
Subject(s) - cfu gm , bone marrow , progenitor cell , medicine , andrology , agar , peripheral blood , in vitro , granulocyte , autologous blood , transplantation , bone marrow transplantation , immunology , stem cell , microbiology and biotechnology , chemistry , surgery , biology , biochemistry , genetics , bacteria
S ummary . Eighteen bone marrows collected from patients without haematological diseases and from normal subjects were tested for the effect of 4 d storage at 4°C on CFU‐C growth. Results indicate that unfractionated bone marrow cells may be stored at 4°C for 4 d with 97%± 8 SEM recovery of the CFU‐C evaluated by the agar culture assay. On the other hand, the same preservation procedure on peripheral blood CFU‐C of 13 normal subjects yielded only 5%± 2 SEM recovery of in vitro growth capacity. The present results have practical implications. They might be exploited to preserve bone marrow CFU‐C for transplantation therapy or laboratory investigation. In contrast this single preservation procedure seems not appropriate for preserving blood CFU‐C.