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Five leucocyte labelling techniques: a comparative in‐vitro study
Author(s) -
ColasLlnhart N.,
Barbu M.,
Gougerot M. A.,
Bok B.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.tb01983.x
Subject(s) - labelling , in vitro , in vivo , pyrophosphate , chemistry , pathology , biochemistry , biology , medicine , enzyme , microbiology and biotechnology
S ummary . During the past years, several authors have used labelled leucocytes to detect inflammatory foci. However, before routine use in man, it is necessary to control the viability of labelled cells. Five leucocyte labelling techniques ( 111 In‐oxine, 111 In‐oxine without extraction, 99m Tc oxine, pyrophosphate 99m Tc, 51 Cr) were compared using the same separation methods, conservation medium, viability assays and migration studies. Electron microscopic studies allowed the assessment of cellular damage induced by the labelling techniques as well as the calculation of the percentage of cells disrupted during preparation. Results obtained in vitro using 111 In‐oxine were not satisfactory and in fact appeared contradictory to those published by the authors using this technique in vivo. Even the best method, pyrophosphate 49m Tc labelling, was not completely atoxic, but the functional behaviour of the leucocytes did not seem affected. While in vitro studies offer much information concerning labelled cells, they cannot predict the in vivo behaviour of these cells.