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Change of pyruvate kinase isozymes from M 2 ‐ to L‐type during development of the red cell
Author(s) -
Takegawa S.,
Fujii H.,
Miwa S.
Publication year - 1983
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1983.00467.x
Subject(s) - pyruvate kinase , isozyme , red cell , basophilic , pyruvate kinase deficiency , fluorescence , microbiology and biotechnology , biology , cell , cell type , red blood cell , biochemistry , chemistry , enzyme , medicine , pathology , glycolysis , physics , quantum mechanics
S ummary . The relationship between erythroid cell maturation and the change of pyruvate kinase (PK) isozymes was studied using fluorescent antibody techniques. The fluorescence of M 2 ‐type PK was strongest in the proerythroblast stage, and then steeply declined with red cell maturation so that only weak fluorescence was seen in orthochromatic erythroblasts. On the other hand, the fluorescence of L‐type PK was seen throughout the maturation of the erythroblasts, with stronger fluorescence in the basophilic or polychromatic erythroblasts than in proerythroblasts.