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Heterogeneity of haemophilia A: a study with three different antisera
Author(s) -
Muller Hein P.,
Van Tilburg Nico H.,
Bertina Rogier M.,
Veltkamp Jan J.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb03918.x
Subject(s) - haemophilia , haemophilia a , immunoradiometric assay , antibody , medicine , antiserum , neutralization , haemophilia b , antigen , immunology , virology , gastroenterology , pediatrics , radioimmunoassay
S ummary . One human and one rabbit antibody against VIII:C—in a fluid‐phase, inhibitor neutralization assay (INA)—and one human antibody—in a solid‐phase, immunoradiometric assay (IRMA)—were used to investigate a group of 59 patients with severe, moderate and mild haemophilia A. Patients were classified as haemophilia A + when the VIII:C/VIIICAG ratio was less than 0.4 while the absolute VIII:C antigen (VIIICAG) value exceeded 0.25 u/ml. Three haemophiliacs (from two pedigrees) were classified as A + in all three assay systems. 50% of the patients were classified as haemophilia A + on the basis of at least one assay. The other 50%, including 66% of the patients with severe haemophilia, were shown to be A‐ by all three assay systems. Combining the data obtained with the three different antibodies four different categories could be distinguished, in addition to the classification based on severity.