Premium
Apparent heterogeneity of human megakaryocyte colony‐ and thrombopoiesis‐stimulating factors: studies on urinary extracts from patients with aplastic anaemia and idiopathic thrombocytopenic purpura
Author(s) -
Kawakita Makoto,
Miyake Takaji,
And Susumu Kishimoto,
Ogawa Makio
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb03912.x
Subject(s) - thrombopoiesis , megakaryocyte , thrombocytopenic purpura , medicine , immunology , thrombopoietin , aplastic anemia , platelet , hematology , urinary system , biology , haematopoiesis , bone marrow , stem cell , genetics
S ummary . Megakaryocyte colony‐stimulating factor (MEG‐CSF) in the urinary extracts from patients with aplastic anaemia (AA) revealed two distinct peaks of activity on Sephadex G‐200 gel filtration with apparent molecular weights of 155 000 and 76 000. Both fractions induced significant thrombocytosis in peripheral blood and megakaryocytosis in the spleen of rats. Heterogeneity of MEG‐CSF was also found in the extracts from the urine of patients with idiopathic thrombocytopenic purpura. The higher molecular weight MEG‐CSF was significantly reduced when the gel filtration was performed under the dissociating conditions. Ion‐exchange chromatography indicated that the higher molecular weight MEG‐CSF had a different charge from the lower molecular weight MEG‐CSF. These results suggest that the apparent heterogeneity of MEG‐CSF is due to interaction of MEG‐CSF with other proteins in the urinary extracts.