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Effect of hydrocortisone on the growth of human bone marrow fibroblasts
Author(s) -
Minguell José J.,
Martianez Jorge,
Walter Tomas
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb03893.x
Subject(s) - fibroblast , stromal cell , bone marrow , hydrocortisone , endocrinology , medicine , cell growth , cell , biology , chemistry , cell culture , microbiology and biotechnology , biochemistry , genetics
S ummary . Fibroblast‐like cells may be grown from human bone marrow. Adherent cultures show a logarithmic phase of growth lasting 6 d. Hydrocortisone (1 x 10 ‐8 M) decreases cell growth in 40‐60% when measured as cell number and DNA synthesis. Fibroblasts bind the hormone by a high affinity, saturable process showing a K d of 2.2 x 10 ‐8 M and an average binding capacity of 92 fmol/mg protein. These findings suggest the involvement of glucocorticoids in the regulation and differentiation processes in bone marrow stromal cells.