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Self‐renewal and differentiation capacity of bone marrow and fetal liver stem cells
Author(s) -
Zucali J. R.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb03892.x
Subject(s) - stem cell , bone marrow , progenitor cell , spleen , fetus , biology , immunology , bone marrow stem cell , cellular differentiation , femur , andrology , microbiology and biotechnology , medicine , pregnancy , genetics , gene , paleontology
S ummary . An operational definition of the pluripotent stem cell (CFC‐S) requires that it have both the capacity for self‐renewal and the potential for differentiation into more than one class of formed blood elements. Because the CFC‐S compartment is heterogeneous, younger stem cells would be expected to be less committed to differentiation and have a higher rate of self‐renewal; whereas, older stem cells would be more committed to differentiation and have a lower rate of self‐renewal. In this study, the self‐renewal capacity versus the differentiation potential of adult bone marrow and fetal liver stem cells were compared. The self‐renewal potential was estimated by determining the number of CFC‐S which develop during growth in the spleen or femur of primary recipients. The differentiation potential was estimated by determining the total number of nucleated cells or committed progenitor cells (GM‐CFU‐C and BFU‐E) which develop during growth in the spleen or femur of primary recipients. In order to circumvent possible differences in self‐renewal or differentiation pressures due to the presence of differing numbers of CFC‐S, an equivalent number of bone marrow and fetal liver CFC‐S were allowed to seed the spleen and femur. While both adult bone marrow and fetal liver stem cells showed an extensive capacity for self‐renewal, fetal liver CFC‐S displayed a greater potential for self‐renewal in both the spleen and femur and at all growth intervals measured when compared to adult bone marrow CFC‐S. In contrast, no differences were seen in the number of nucleated cells or committed stem cells found per CFC‐S when comparing adult bone marrow and fetal liver stem cells.