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B‐CLL cells express true endogenous E receptor after culture with T‐cells and mitogens
Author(s) -
Worman C. P.,
Cawley J. C.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb03882.x
Subject(s) - peripheral blood mononuclear cell , monoclonal antibody , microbiology and biotechnology , biology , immunology , receptor , antibody , cell culture , monoclonal , in vitro , genetics
S ummary . Culture studies of peripheral blood mononuclear cells from seven typical cases of B‐cell chronic lymphatic leukaemia (CLL) showed that, in the presence of PHA or Con A and ≧20% autologous or allogeneic T‐cells, CLL cells express true sheep erythrocyte (E) receptor after 2‐5 d, but fail to stain with a panel of anti‐T‐cell sera. In contrast to similarly cultured hairy cells (HCs), the cultured CLL cells continue to express light‐chain‐restricted surface immunoglobulin (SIg) and show no change in their morphology. The continued SIg positivity and staining with a panel of monoclonal antibodies indicated that the high proportion of E + cells could not be attributed to the outgrowth of normal T‐cells or to mitogen‐induced non‐specific stickiness. Repeated addition of supernatants from cultured T‐cells caused no phenotypic changes and therefore excluded the possibility of shedding and passive acquisition of E receptor. The points of similarity and difference between the responses of CLL cells and HCs cultured in a like manner are briefly considered in relation to the possible lineages of these two cell types.