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A SINGLE‐STEP BACTERIAL BINDING ASSAY FOR THE CLASSIFICATION OF CELL TYPES WITH SURFACE ANTIGEN‐DIRECTED MONOCLONAL ANTIBODIES
Author(s) -
UchanaskaZiegler Barbara,
Wernet Peter,
Ziegler Andereas
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb03871.x
Subject(s) - monoclonal antibody , antigen , microbiology and biotechnology , cell , antibody , chemistry , cell membrane , cell culture , biology , immunology , biochemistry , genetics
S ummary . A rapid and cheap single‐step bacterial‐binding assay for the detection of cell‐membrane molecules is described. It offers the opportunity to correlate antigen expression with cell morphology and cytochemistry and gives an indicagtion of the quantitiy of a cell surface molecule. The value of this procedure is demonstrated by the analysis of spontaneously grwoing and differentiating cultures of the human myeliod leukaemia line HL‐60 with monoclonal antibodies.