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Serological studies in patients on platelet‐ and granulocyte‐substitution therapy
Author(s) -
Pegels J.G.,
Bruynes E.C.E.,
Engelriet C.P.,
Kr. vondem Borne A.E.G.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb03861.x
Subject(s) - platelet , immunofluorescence , serology , medicine , granulocyte , paraformaldehyde , immunology , antibody , isoantibodies , bone marrow , pathology
S ummary . A serological follow‐up study was undertaken in 47 patients with bone‐marrow failure, who were repeatedly transfused with random donor granulocytes and/or platelets. Sera, obtained at regular intervals, were investigated in the leucoagglutination test, the lymphocytotoxic test and the immunofluorescence test on paraformaldehyde‐fixed platelets, granulocytes and lymphocytes. The frequency of alloimmunization was high (73%). Not only HLA antibodies, but also blood‐cell‐specific alloantibodies were detected in the sera of the alloimmunized patients, e.g. lymphocyte‐specific, platelet‐specific and granulocyte‐specific antibodies. The immunofluorescence test on platelets was also used as a crossmatch, and when this test was positive it was always found that after platelet transfusion the increment value was nil.