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Cell lines derived from a human myelomonocytic leukaemia
Author(s) -
Bradley T. R.,
Pilkington G.,
Garson M.,
Hodgson G. S.,
Kraft N.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb02823.x
Subject(s) - cell culture , peripheral blood , monocyte , immunology , virus , virology , medicine , biology , cancer research , genetics
S ummary . Two cell lines have been isolated from separate samples, taken at the same time, from the peripheral blood of a case of myelomonocytic leukaemia. One of these (CESS‐B) is a typical B cell line obtained after treatment of the peripheral blood white cells with Epstein‐Barr virus (EBV). The second line (RC‐2) is a monocyte‐macro‐phage type, originally isolated by 5 months continuous culture in the presence of colony stimulating factor (CSF). Subsequently, after further culture, this line has become autonomous (RC‐2A), no longer requiring CSF for maintenance of proliferation, whilst retaining the characteristics of the original line.