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Enzymehistochemical staining of Tμ lymphocytes for glycyl‐proline‐4‐methoxy‐beta‐naphthylamide‐peptidase (DAP IV)
Author(s) -
Feller A. C.,
Heijnen C.J.,
Ballieux R. E.,
Parwaresch M. R.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb02775.x
Subject(s) - proline , receptor , microbiology and biotechnology , staining , t lymphocyte , lymphocyte , enzyme , chemistry , immunology , biology , pathology , biochemistry , medicine , antigen , amino acid
S ummary . Human Tμ lymphocytes are detected in a histochemical reaction with glycyl‐proline‐4‐methoxy‐β‐naphthylamide‐peptidase (DAP IV; EC 3.4.14.4). DAP IV occurs in 59.4 ± 8.2% of human T lymphocytes in blood and marrow. Direct biochemical assays were in accord with the cytochemical findings. DAP IV‐positive lymphocytes accounted for 89.5 ± 5.3% of the Tμ and 13.0 ± 6.4% of the Tγ fraction. Tγ fraction which had 93.7 ± 0.6% of T‐lymphocytes bearing Fc receptor for IgG contained 11.3 ± 8.3% T‐lymphocytes bearing Fc receptor for IgM.