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Quantitation of RNA‐dependent platelet DNA polymerase in patients with myeloproliferative disorders
Author(s) -
Strayer David R.,
Brodsky Isadore,
Caranfa Mary Jo,
Gillespie David H.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb01948.x
Subject(s) - polycythaemia , myelofibrosis , platelet , myeloproliferative disorders , essential thrombocythemia , polycythemia vera , polymerase chain reaction , medicine , myeloid , immunology , biology , bone marrow , gene , biochemistry
Platelets from 28 patients with the myeloproliferative diseases (MPD) polycythaemia vera (9), essential thrombocythaemia (6), myelofibrosis with myeloid metaplasia (5) and chronic myelogenous leukaemia (8) were examined for an RNA‐dependent DNA polymerase activity using standardized conditions permitting highly reproducible quantitation. Low levels of activity were detected in platelets of normal individuals, but platelets of nearly all MPD patients (25/28) possessed higher levels. The polymerase activity correlated with diagnosis ( P = 0.001) and did not correlate with platelet counts ( P >0.2). Quantitation of this RNA‐dependent DNA polymerase activity may be a useful parameter in the diagnosis of myeloproliferative disorders.