Premium
Precipitating anti‐VIII:C antibodies in two patients with haemophilia A
Author(s) -
Lavergne JeanMaurice,
Meyer Dominique,
Girma JeanPierre,
Larrieu MarieJosé
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb01898.x
Subject(s) - cryoprecipitate , precipitin , haemophilia a , haemophilia , antibody , immunoradiometric assay , chemistry , von willebrand factor , microbiology and biotechnology , cryoglobulins , immunodiffusion , coagulopathy , radioimmunoassay , immunology , medicine , biochemistry , biology , platelet , pediatrics
S ummary . IgG from the plasmas of two haemophilia A patients with anti‐VIII:CAg antibodies (1000 and 200 u/ml) was isolated and labelled with 125 I. The specific labelled anti‐VIII:CAg IgG was further purified by binding to and elution from immobilized factor VIII/von Willebrand factor (F.VIII/vWF). When studied by immunodiffusion and autoradiography, both antibodies gave a precipitin line with normal plasma, serum, cryoprecipitate, purified F.VIII/vWF and the plasmas of two patients with haemophilia A + . No precipitin line was observed with the plasmas of 11 patients with haemophilia A − or four patients with severe von Willebrand's disease. Levels of VIII:CAg obtained by radioelectroimmunoassay were in agreement with those obtained by immunoradiometric assay. This study demonstrates that, contrary to previous evidence, human anti‐VIII:CAg antibodies are precipitating as well as neutralizing when studied by highly sensitive techniques.