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Immuno‐histological analysis of bone marrow involvement in lymphoproliferative disorders
Author(s) -
Pizzolo G.,
Chilosi M.,
Cetto G. L.,
FioreDonati L.,
Janossy G.
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb01894.x
Subject(s) - lymphoproliferative disorders , bone marrow , medicine , pathology , hematology , immunology , lymphoma
S ummary . Cryostat sections from bone marrow biopsies submerged in gum‐sucrose solution and snap‐frozen can be stained with various antisera to detect both the membrane and cytoplasmic antigens on normal and malignant populations in their tissue environments. This technique seems to be the method of choice to analyse the degree of BM involvement in cases of non‐Hodgkin's lymphoma where conventional histology provides equivocal results. The uniform expression of only one type of light chain in B lymphoma and the analysis of the T cell infiltrate in T‐CLL using monoclonal antibodies is demonstrated.