Premium
Specific Enhancement of Mouse CFU‐E by Mouse Transferrin
Author(s) -
Rich Ivan N.,
Sawatzki Gunter,
Kubanek Bernhard
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.tb07265.x
Subject(s) - transferrin , erythropoietin , bone marrow , granulocyte , erythropoiesis , microbiology and biotechnology , biology , ratón , macrophage , cell culture , immunology , chemistry , biochemistry , endocrinology , medicine , anemia , in vitro , genetics
S ummary . Pure human and mouse transferrins were prepared by a chromatographic procedure and their effect on the growth of early (BFU‐E) and late (CFU‐E) erythropoietic precursors in mouse bone marrow is described. In the presence of optimal erythropoietin concentrations mouse bone marrow cells have a greater specificity for mouse transferrin (950 CFU‐E colonies/10 5 cells) than human transferrin (650 CFU‐E colonies/10 5 cells). Optimal transferrin concentrations for both human and mouse transferrins were 1.3 × 10 −13 M and 1.3 × 10 −10 M corresponding to between 7.8 × 10 7 and 7.8 × 10 10 molecules/ml of culture. These concentrations are in excess of that calculated on a theoretical basis. Neither erythropoietic burst nor granulocyte/macrophage colony formation exhibited a dose dependent relationship for any of the transferrins employed, although higher colony numbers were obtained with mouse transferrin compared to human transferrin.