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Monoclonal Antibody to Human Platelet Glycoprotein I I. IMMUNOLOGICAL STUDIES
Author(s) -
McMichael A. J.,
Rust N. A.,
Pilch J. R.,
Sochynsky R.,
Morton J.,
Mason D. Y.,
Ruan C.,
Tobelem G.,
Caen J.
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.tb07258.x
Subject(s) - thrombasthenia , platelet , bernard–soulier syndrome , monoclonal antibody , glycoprotein , antigen , immunoprecipitation , microbiology and biotechnology , platelet membrane glycoprotein , antibody , glycoprotein ib , chemistry , glanzmann's thrombasthenia , immunology , biology , platelet aggregation
S ummary . A monoclonal hybridoma antibody specific for platelet glycoprotein I complex is described. The nature of the antigen was determined by demonstration that it was chymotrypsin sensitive and gave a peak at 150000 daltons on SDS‐PAGE after immunoprecipitation. The expression of the antigen is restricted to platelets and megakaryocytes with at least 1.6 × 10 4 molecules of antigen per platelet. The antibody failed to bind to platelets from patients with Bernard Soulier syndrome, where there is known to be a deficiency of glycoprotein Ib/Is expression. Binding to platelets from patients with Glanzmann's thrombasthenia was normal.