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Entry of Malaria Parasites into Resealed Ghosts of Human and Simian Erythrocytes
Author(s) -
Dluzewski A. R.,
Rangachari K.,
Wilson R. J. M.,
Gratzer W. B.
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.tb07201.x
Subject(s) - plasmodium knowlesi , lysis , malaria , plasmodium falciparum , biology , simian , cytosol , cytolysis , tonicity , virology , parasite hosting , plasmodium (life cycle) , red blood cell , microbiology and biotechnology , immunology , biochemistry , in vitro , virus , plasmodium vivax , cytotoxicity , world wide web , computer science , enzyme
S ummary . Whereas‘white’ghosts, conventionally prepared by hypotonic lysis and washing are resistant to invasion by malaria parasites, cells lysed at high haematocrit and resealed without separating the membranes from the cytosol are invaded with high efficiency (not less than one‐third the frequency of intact cells). Similar results were obtained with human and monkey cells, exposed respectively to Plasmodium falciparum and P. knowlesi. Microspectrophotometry of individual cells was used to ascertain that the susceptibility to invasion of lysed, resealed cells was not related to its haemoglobin content.