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In Vitro Assay of Erythropoietin in Fetal Mouse Liver Cultures
Author(s) -
Brandan N. C.,
Cotes P. M.,
Espada J.
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.tb02815.x
Subject(s) - erythropoietin , in vitro , fetus , transferrin , medicine , thymidine , endocrinology , stimulation , incubation , erythropoiesis , microbiology and biotechnology , chemistry , biology , biochemistry , anemia , pregnancy , genetics
S ummary . Iron bound to human transferrin but not apotransferrin, increases the effect of erythropoietin in stimulating incorporation of H‐thymidine into DNA in fetal mouse liver cells in vitro. The effect of erythropoietin, with or without transferrin‐iron is blocked by pre‐incubation of the erythropoietin with rabbit anti erythropoietin serum. Human sera contain factors in addition to erythropoietin and transferin‐iron which may modify the stimulation of incorporation of H‐thymidine into fetal mouse liver DNA induced by erythropoietin. Heat treatment of sera at 56°C for 30 min does not necessarily destroy these factors. Acid heat treatment of sera (pH 5.5 and 100°C for 5 min) may destroy inhibitory factors and can result in an apparent increase in serum erythropoietin activity assessed in this fetal mouse liver system.