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In Vitro Assay of Erythropoietin in Fetal Mouse: Liver Cultures
Author(s) -
Brandan N. C.,
Cotes P. M.,
Espada J.
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.tb02814.x
Subject(s) - erythropoietin , dna synthesis , in vitro , thymidine , bioassay , dna , stimulation , in vivo , fetus , incubation , biology , antiserum , uridine , microbiology and biotechnology , medicine , endocrinology , biochemistry , chemistry , rna , antibody , immunology , pregnancy , genetics , gene
S ummary . In a 13 d fetal mouse liver tissue culture system for assay of erythropoietin, stimulation of incorporation of H‐thymidine and of 125 I‐iodo‐2′‐deoxy uridine into DNA and of 59 Fe iron into haem were compared as metameters of erythropoietin effect. With both DNA and haem tracers, log‐dose response curves given by two preparations of human urinary erythopoietin purified to different extents (respectively 2 and 1300IU, by bioassay in vivo , per mg of protein) were essentially identical at low dose levels although at high dose levels the less pure preparation decreased stimulation of both DNA and haem synthesis. Pre‐incubation of erythropoietin with an antiserum to human erythropoietin abolished the effects of the hormone on both DNA and haem synthesis.