Premium
Liposome‐encapsulated Desferrioxamine in Experimental Iron Overload
Author(s) -
Lau E. H.,
Cerny E. A.,
Rahman Y. E.
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.tb02679.x
Subject(s) - liposome , chemistry , microgram , ferritin , galactocerebroside , parenchyma , biochemistry , pathology , medicine , in vitro , myelin , oligodendrocyte , central nervous system
S ummary . We have encapsulated desferrioxamine (DF) in multilamellar liposomes (ML) and unilamellar liposomes (UL). Liposomes were prepared either with or without a glycolipid, i.e. galactocerebroside (GC). The average diameter of ML was 0.5 μm, and that of UL was 0.08 μm. Less than 5% of DF leaked out from the liposomes after incubation in mouse plasma for 6 h. 59 Fe‐ferritin and 59 Fe‐labelled heat damaged erythrocytes ( 59 Fe‐DRBC) were administered to normal and hyper‐ transfused mice as models of iron overload. Ferritin was used to label liver parenchymal cells and DRBC to label the Kupffer cells of the liver. A single injection of ML or UL with or without galactocerebroside into normal and hypertransfused mice enhanced from 3‐ to 15‐fold the urinary excretion of radioiron from 59 Fe‐ferritin and from 59 Fe‐DRBC injected mice. For both the normal and hypertransfused mice, liposomes containing GC removed more 59 Fe radioactivity from 59 Fe‐ferritin injected mice than liposomes without the glycolipid; whereas liposomes without GC removed more 59 Fe radioactivity from mice receiving 59 Fe‐DRBC. Thus GC‐liposomes may have a higher affinity for parenchymal cells of the liver, whereas liposomes without the glycolipid may have a higher uptake by the Kupffer cells.