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MYELOFIBROSIS AND PROSTAGLANDINS: EFFECT OF PROSTAGLANDIN E 1 ON COLONY‐FORMING CELLS (CFU‐GM)
Author(s) -
Aglietta Massimo,
Piacibello Wanda,
Gavosto Felice
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.00167.x
Subject(s) - cfu gm , prostaglandin e , in vitro , myelofibrosis , prostaglandin , colony forming unit , biology , haematopoiesis , endocrinology , medicine , andrology , chemistry , immunology , bone marrow , stem cell , biochemistry , microbiology and biotechnology , bacteria , genetics
Semisolid agar cultures of committed granulocyte monocyte precursors (CFU‐GM) make it possible to study the regulation of normal and abnormal stem cells. In vitro growth of CFU‐GM is dependent on the presence of specific stimulators, called CSA (colony stimulating activity) (Metcalf, 1977; Broxmeyer & Moore, 1978). The influence of CSA on CFU‐GM may be inhibited by prostaglandins of the E series (Broxmeyer & Moore, 1978; Kurland et al , 1978). Recently it has been demonstrated that chronic granulocytic leukaemia (CGL) CFU‐GM are insensitive to prostaglandin E 1 (PGE 1 ) over a wide range of concentrations that are inhibitory to normal CFU‐GM development (Aglietta et al , 1980; Pelus et al , 1980; Taetle & Koessler, 1980). We report the effect of PGE 1 on CFU‐GM from patients with idiopathic myelofibrosis (MF).