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Erythroid Colony Growth in Vitro from Human Peripheral Blood Null Cells: Evidence for Regulation by T‐Lymphocytes and Monocytes
Author(s) -
Reid C. D. L.,
Baptista Luisa C.,
Chanarin I.
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.00155.x
Subject(s) - in vitro , peripheral blood , immunology , null cell
S ummary. Colony assays in methylcellulose of primitive erythroid precursors (BFU‐E) were carried out from the null cell fraction of normal human peripheral blood lymphoid cells. There was little proliferation or maturation of BFU‐E as assessed by both the number and the size of colonies formed, when null cells alone were cultured. Culture of null cells with up to 8 × 105 autologous T‐lymphocytes per ml led to considerable stimulation of colony growth and maturation. Culture of null cells with peripheral blood monocytes also resulted in the induction of BFU‐E growth, although the response was inferior to that seen with T‐cells. Co‐culture of null cells together with both T‐cells and monocytes resulted in a uniformly greater response than with either alone, and this was shown to be due to a positive interaction between these two cell types.