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Characterization of a Developmentally Early Macrophage Progenitor Found in Normal Mouse Marrow
Author(s) -
Baines Paul,
Bol Simon,
Rosendaal Martin
Publication year - 1981
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1981.00147.x
Subject(s) - bone marrow , macrophage , spleen , progenitor cell , biology , immunology , granulocyte , andrology , microbiology and biotechnology , stem cell , medicine , in vitro , biochemistry
S ummary. In tissue culture high proliferation potential colony forming cells (HPP‐CFC, Bradley & Hodgson, 1979) formed large colonies in which macrophages predominated. Granulocyte/macrophage colony forming cells (GM‐CFC, Bradley & Metcalf, 1966) formed smaller colonies of granulocytes and macrophages. We compared HPP‐CFC from normal adult mouse bone marrow with GM‐CFC from the same source. We found that HPP‐CFC differed from GM‐CFC in the following ways: (1) HPP‐CFC formed larger colonies containing more than 5 × 10 4 cells. (2) They required spleen conditioned medium (Metcalf & Johnson, 1978) as well as pregnant mouse uterine extract (PMUE, Bradley et al , 1971) as stimulants. (3) Only 9% of HPP‐CFC were killed by the phase specific drug hydroxyurea. 36–55% of GM‐CFC were killed by the same treatment. (4) The modal density of HPP‐CFC was 1·070 g/cm 3 . That of GM‐CFC was 1·080 g/cm 3 .