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Effect of Different Resuspension Media on the Post‐thaw Characteristics of Frozen Blood
Author(s) -
Amer Kamal A.,
Pepper Duncan S.,
Prowse Christopher V.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb08718.x
Subject(s) - haemolysis , potassium , saline , glycerol , chemistry , blood bank , phosphate buffered saline , hematocrit , cryopreservation , andrology , food science , chromatography , biology , medicine , biochemistry , immunology , fishery , embryo , organic chemistry , medical emergency
Summary. Red blood cells frozen by the low‐glycerol fast‐freezing technique were thawed, deglycerolized and resuspended in various media. The use of ACD‐saline for resuspension markedly reduced in vitro haemolysis such that the red cells could be transfused up to 5 d after thawing. At this time the cells contained satisfactory levels of potassium and organic phosphates, while bacterial contamination was negligible. For the past 4 years we have operated a small bank of previously frozen red blood cells which were thawed and resuspended in this way. Over 3500 units have been transfused after 1–5 d post‐thaw storage without untoward effects.