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Globin Chain Electrophoresis: a New Approach to the Determination of the G γ/ A γ Ratio in Fetal Haemoglobin and to Studies of Globin Synthesis
Author(s) -
Alter Blanche P.,
Goff Sabra C.,
Efremov Georgi D.,
Gravely Marsha E.,
Huisman Trrus H. J.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb08706.x
Subject(s) - globin , electrophoresis , fetal hemoglobin , gel electrophoresis , chemistry , polyacrylamide gel electrophoresis , hemoglobin , beta globulins , microbiology and biotechnology , biochemistry , chromatography , biology , gamma globulin , fetus , antibody , enzyme , genetics , pregnancy
Summary. Separation of globin chains by electrophoresis provides a simple and rapid method for the determination of the G γ/ A γ ratio in human fetal haemoglobin, and of biosynthetic rates of the globin chains. Whole haemolysates were analysed by electrophoresis on polyacrylamide gels in urea, acetic acid and Triton X‐100. Electrophoresis of haemolysates from newborn infants led to four bands: A γ, G γ, β and α. The identity of these bands was indicated by examination of haemoglobins of known globin chain composition. In 15 samples, the % G γ was similar by Triton gels and by amino acid analysis of the γCB‐3 peptide. Some mutant globin chains were also separable with the electrophoretic technique. Triton gel electrophoresis provides rapid analysis of very small amounts of haemoglobin, and permits examination of globin chain composition as well as globin synthetic ratios.