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Inhibition of Normal Human Granulopoiesis in Vitro by Non‐B Non‐T Lymphocytes
Author(s) -
Morris T. C. M.,
Vincent P. C.,
Sutherland Ruth,
Hersey P.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb07176.x
Subject(s) - granulopoiesis , peripheral blood mononuclear cell , population , biology , microbiology and biotechnology , in vitro , bone marrow , lymphocyte , immunology , haematopoiesis , stem cell , biochemistry , medicine , environmental health
S ummary Normal non‐adherent mononuclear cells were shown to inhibit colony formation by normal human marrow cells cultured for 7 d in semi‐solid agar. Inhibition was the same using cells from the marrow donor or from an unrelated normal subject, and was shown to be dose‐dependent over the range of 4 × 10 5 to 6 × 10 3 mononuclear cells per 1 × 10 5 marrow cells plated. Inhibition was not seen in 14 d cultures, and it is postulated that colony‐forming cells sensitive to lymphocyte inhibition belonged to the population known to give rise to colonies after 7 d in culture. Cell fractionation studies showed that inhibition was due to non‐B non‐T lymphocytes, purified B cells or T cells being neither inhibitory nor stimulatory. Inhibition was only shown with intact viable lymphocytes and it was not possible to extract inhibitory activity from the cells, or to produce inhibition by media conditioned by lymphocytes. The effect was apparently due to a direct action on colony‐forming cells in the marrow and was not due to inhibition of colony stimulating activity (CSA) production, or to absorption or inactivation of CSA. These results emphasize the need to include appropriate controls when looking for possible cell‐mediated inhibitors in disease states, particularly when 7 d cultures are used.