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Functional Restoration of ACD‐Blood by Pyridoxal 5′‐Phosphate
Author(s) -
Maeda Nobuji,
Kon Kazunori,
Sekiya Misuzu,
Shiga Takeshi
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb07166.x
Subject(s) - pyridoxal phosphate , pyridoxal 5 phosphate , chemistry , phosphate , pyridoxal , biochemistry , oxygen , oxygen transport , red blood cell , cofactor , enzyme , organic chemistry
S ummary Successful application of pyridoxal 5′‐phosphate (PLP) to restore the oxygen transport function of ACD‐stored blood is described. PLP is readily incorporated into ACD‐erythrocytes by both carrier‐mediated transport (in which ATP may participate) and passive diffusion. Plasma proteins (up to 2·5%) and inorganic phosphate (up to 40 m m ) do not affect the incorporation of PLP, though more than 25 m m inorganic phosphate is necessary for the maintenance of ATP levels. Increasing the PLP concentration and/or decreasing the packed cell volume in the medium, increases the incorporation of PLP. Incubation of erythrocytes with PLP at pH 7·0 is most suitable for incorporation of PLP and the maintenance of ATP levels. PLP incorporated into erythrocytes restores the oxygen transport function of the ACD‐erythrocytes, though decreased haem‐haem interaction is observed. A procedure for the clinical application of PLP‐loaded erythrocytes is suggested.