Premium
Variation in Sensitivity of DNA Synthesis to Ara‐C in Acute Myeloid Leukaemia
Author(s) -
Harris Adrian L.,
GrahameSmith D. G.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb07157.x
Subject(s) - dna synthesis , dna , microbiology and biotechnology , myeloid leukaemia , chemistry , in vitro , biology , biochemistry , cancer research
S ummary The sensitivity of DNA synthesis to ara‐C was measured in vitro in intact myeloblasts from patients with acute myeloid leukaemia (AML). Ara‐C concentrations of 1 n m to 100 μ m were used and ara‐CTP production measured at the same concentrations. Maximum inhibition of DNA synthesis was 95% in all patients. At ara‐C concentrations above 1 μ m there was little variation in sensitivity of DNA synthesis but at concentrations below 300 μ m there was wide variation in inhibition. Ara‐CTP concentrations associated with 50% inhibition of DNA synthesis ranged from 0·034 to 1·0 pmol/10 6 cells. Variation in sensitivity of DNA synthesis to ara‐C could not be explained by differences in ara‐CTP production alone. Concentrations of 100 n m to 1 μ m ara‐C in vivo may be desirable to produce greater than 50% inhibition of DNA synthesis in all patients’myeloblasts.