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Assessment of Complement Binding by Anti‐D and Anti‐M Antibodies Employing Labelled Antiglobulin Antibodies
Author(s) -
Freedman J.,
Massey A.,
Chaplin H.,
Monroe M. C.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb07150.x
Subject(s) - antibody , incubation , immunology , coombs test , umbilical cord , chemistry , in vitro , microbiology and biotechnology , medicine , biochemistry , biology
S ummary The presence of small amounts of C3d on freshly obtained normal red blood cells (RBC) was demonstrated by a radio‐labelled antiglobulin technique; this increased 1·5‐fold after incubation in fresh normal serum. No significant further increase in bound C3d was demonstrated for cDE/cDE, CDe/CDe, ‐D‐/‐D‐ RBC maximally sensitized with any of three potent anti‐D antibodies. When anti‐c, anti‐D and anti‐E antibodies were used in combination to sensitize cDE/cDE RBC, bound C3d increased by approximately 22%; however, a similar increase occurred with cde/cde RBC and was therefore considered non‐Rh specific. Umbilical cord RBC from four infants severely affected by anti‐D haemolytic disease of the newborn did not exhibit more bound C3d than cord RBC from normal controls. One serum containing IgM anti‐M bound a small amount of complement in vitro ; two IgG anti‐M sera did not.